Hormones

DHEA (dehydroepiandrosterone) is a hormone produced by the adrenal glands and serves as a precursor to sex hormones. Measuring DHEA in saliva reflects the body’s active hormone levels, offering insight into immediate DHEA production. This measurement is helpful for evaluating adrenal health, aging, and the risk of chronic diseases.

DHEA

The estrogen/progesterone (E/P) ratio is a measurement that indicates the balance between two essential hormones in the body — estrogen (E) and progesterone (P). Both hormones are crucial for female reproductive health, including the regulation of menstrual cycles, fertility, mood, and overall wellness. Maintaining an optimal ratio promotes hormonal balance, while imbalances may lead to various health concerns. This ratio naturally varies throughout the menstrual cycle and can be affected by factors such as age, lifestyle, and medical conditions.

Estrogen/Progesterone ratio

Prolactin is a hormone produced by the pituitary gland primarily associated with lactation in women. It also plays key roles in fertility, immune system regulation, and sexual satisfaction. Elevated prolactin levels can cause symptoms such as irregular menstrual cycles or infertility, while low levels may affect milk production after childbirth.

Prolactin

Progesterone is a hormone that plays a crucial role in the female reproductive system, mainly produced in the ovaries after ovulation. It prepares the uterus for pregnancy and helps sustain it if conception occurs, while a decline in progesterone triggers menstruation. Beyond reproduction, progesterone affects sexual desire, mood, and bone health. During pregnancy, the placenta produces progesterone to support fetal development and prepare for breastfeeding. In men, progesterone is present at lower levels and contributes to sperm production.

Progesterone

The Testosterone/Cortisol ratio reflects the balance between anabolic (building) and catabolic (breaking down) processes in the body. Testosterone supports muscle growth, energy, and recovery, while cortisol is a stress hormone that can promote tissue breakdown when chronically elevated. A healthy ratio may indicate good resilience, recovery capacity, and hormonal balance, whereas a low ratio may suggest high stress load, overtraining, or hormonal imbalance. This marker is particularly relevant for athletes, individuals under chronic stress, or those with fatigue-related symptoms. It helps provide context to both testosterone and cortisol values when interpreted together.

Testosterone/Cortisol ratio

Thyroid Stimulating Hormone (TSH) is a hormone produced by the pituitary gland that regulates thyroid function. It signals the thyroid gland to produce thyroid hormones, which are essential for metabolism, energy regulation, and growth. TSH levels provide important information for diagnosing thyroid disorders and assessing whether the thyroid is functioning properly.

Free testosterone refers to the fraction of testosterone in the bloodstream that is not bound to proteins like sex hormone-binding globulin (SHBG) or albumin. Unlike bound testosterone, free testosterone is biologically active and readily available to interact with cells, making it a key indicator of hormonal activity in the body. It plays a vital role in regulating libido, energy levels, muscle strength, and mood in both men and women.

Testosterone (free)

Sex hormone-binding globulin (SHBG) is a glycoprotein primarily produced by the liver that binds and transports sex steroids—mainly testosterone and estradiol—in the bloodstream. By binding these hormones, SHBG controls how much is biologically available to tissues. Higher SHBG levels typically decrease the free (unbound) fraction of testosterone and estradiol, while lower SHBG levels increase it. Due to this regulatory function, SHBG plays a key role in hormonal balance for both men and women and is often measured alongside total testosterone and estradiol to help with interpretation.

Sex hormone-binding globulin (SHBG)

Cortisol is the primary glucocorticoid hormone produced by the adrenal glands and is commonly known as the “stress hormone.” It plays a vital role in regulating metabolism, immune response, blood pressure, and the body’s ability to handle physical and emotional stress. Cortisol follows a daily cycle, peaking early in the morning to help promote alertness and gradually decreasing throughout the day. Measuring total cortisol in the blood reflects the combined levels of free and protein-bound cortisol, offering insight into adrenal function and overall hormone balance. Abnormal cortisol levels may be linked to conditions such as adrenal insufficiency, Cushing’s syndrome, chronic stress, or metabolic disorders.

Cortisol (total)

Follicle-Stimulating Hormone (FSH) is a hormone produced by the pituitary gland that plays a crucial role in reproduction for both men and women. In women, FSH prompts the ovaries to mature egg follicles, while in men, it supports sperm production. FSH levels fluctuate throughout a woman’s menstrual cycle and are vital for fertility and overall reproductive health.

Free Cortisol refers to the unbound, biologically active form of cortisol circulating in the body. Unlike total cortisol, which includes both bound and unbound hormone, free cortisol represents the fraction that is available to tissues and can directly influence stress response, metabolism, immune function, and energy levels. Measuring free cortisol provides a more accurate reflection of how the body is responding to physical and emotional stress. It is commonly assessed in urine or saliva samples and is particularly relevant in evaluating adrenal function and circadian rhythm imbalances.

Cortisol (free)

Melatonin is a hormone mainly produced by the pineal gland in the brain. It plays a crucial role in regulating the body’s internal clock, including the sleep-wake cycle. Melatonin levels naturally increase in the evening, reach their peak overnight, and decrease by morning, helping signal the body to get ready for rest. It also supports other functions such as immune response and antioxidant protection.

Melatonin

DHEA-S (dehydroepiandrosterone sulfate) is the more stable and abundant form of DHEA found in the blood. Measuring DHEA-S levels provides a longer-term indicator of DHEA status, aiding in the assessment of hormonal balance, adrenal gland function, and age-related hormonal changes.

DHEA-S

Free Thyroxine (fT4) is a vital thyroid hormone produced by the thyroid gland, playing a crucial role in metabolism, brain development, and bone health. It acts as the precursor to the active hormone fT3. Measuring fT4 offers insight into thyroid function by reflecting the hormone that is directly released and available for conversion.

Free T4 (fT4)

Estrogen is a primary sex hormone found in both women and men, though at varying levels. In women, it supports the development and regulation of the reproductive system, menstrual cycle, and secondary sexual characteristics, while also affecting bone health, skin, and mood. In men, it contributes to libido, erectile function, and sperm production. It is primarily produced in the ovaries for women and the testes for men. Estrogen levels naturally fluctuate during puberty, menstrual cycles, pregnancy, and menopause. Imbalances can lead to menstrual irregularities, mood changes, and other health issues.

Estrogen (estradiol)

Testosterone is a hormone that plays a crucial role in both male and female health. In men, it is primarily produced in the testes and is vital for the development of male reproductive tissues, promoting muscle growth, body hair, libido, and mood regulation. In women, testosterone is produced in smaller quantities by the ovaries and adrenal glands, where it supports bone strength, ovarian function, and sexual desire.

Testosterone (total)

Free Triiodothyronine (fT3) is a measurement that indicates the level of the active thyroid hormone available in the body. As one of the two main thyroid hormones, fT3 plays a crucial role in regulating metabolism, heart rate, and body temperature. Unlike total T3, fT3 represents the unbound hormone that is immediately available for use, making it an important marker of thyroid function.

Free T3 (fT3)

Luteinizing Hormone (LH) is a hormone produced by the anterior pituitary gland that plays a key role in reproductive health for both men and women. In women, LH triggers ovulation and supports the menstrual cycle by stimulating progesterone production. In men, it promotes testosterone production in the testes. Maintaining balanced LH levels is important for proper hormonal regulation and reproductive function.

Luteinizing Hormone

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About Cortisol
Cortisol is a stress hormone that is released when we are exposed to physical or mental stress. Cortisol is produced in the adrenal glands and helps you perform better physically and mentally in situations that the body perceives as acute stress. In the distant past, stress was a natural reaction to protect yourself from predators, whereas nowadays stress takes on a different form. Today, our stress comes from a modern setting, such as stress about paying the bills.
 
The stress we experience in today’s society often becomes chronic stress. This means high levels of cortisol building up in the body for long periods of time. This causes high levels of cortisol which is harmful and dangerous to the body, resulting in high blood pressure and high blood sugar which can cause type-2 diabetes.
 
However, too little cortisol in the body is not good either. If the cortisol levels become really low, the urine excretes large amounts of sodium, which can lead to life-threatening conditions. 
 
It is important to know if you are at a risk of being affected by any of these conditions. By taking GetTested’s Cortisol test, you can get an indication of your cortisol levels and take adequate measures to prevent cortisol disorders. 
The function of cortisol
Cortisol has a variety of functions in our body. In acute stressful situations, cortisol secretion helps you perform better, both physically and mentally. Cortisol is also important for the movement of the intestines and controls the body’s metabolism of sugar, fat and protein. With its anti-inflammatory effect, cortisol counteracts the function of the immune system, which makes us more susceptible to infections.
Your cortisol level is not always related to your stress level. Physical activity also increases cortisol secretion in the body, but unlike secretion during stress, cortisol instead reduces inflammation that occurs on the muscles. In the medical world, cortisol is best known for its anti-inflammatory and muscle-degrading effects in the treatment of inflammatory diseases, and this is then treated by adding cortisone.
Cortisol also controls the immune system and reduces swelling in allergies and insect bites. Therefore, Cortisol is a vital hormone for our wellbeing. 
Cortisol fluctuations 
Cortisol levels should be at the highest in the morning and lowest at night. The metabolism of fats, sugars and proteins is all controlled by the cortisol.  The amount of cortisol itself depends on what you do and how much stress you put on yourself. Stress can raise the cortisol levels, as well as being injured, having fever, infections or too high adrenaline. 
Symptoms of high cortisol:
In the long run, however, high levels are harmful to the body and with high levels of cortisol for a long time, fat burning decreases and impairs insulin sensitivity, which in the long run can lead to obesity and type-2 diabetes.

Fatigue
Low libido
Weight gain
Difficulty sleeping
High blood pressure
Poor immune function

Symptoms of low cortisol:
Low levels of cortisol can lead to menstrual disorders, muscle weakness and general mental illness, as well as cortisol inhibiting inflammation. In more rare and extreme cases, low cortisol can lead to adrenal insufficiency (also known as Addison’s disease). In such cases, the body does not make enough hormones. Diseases like Addison’s disease can cause life-threatening conditions and usually need to be medicated, but “Milder” conditions still cause a huge stress on the body and mind and should not be left untreated.
The most common symptoms of low cortisol are: 

Menstrual disorders
Fatigue
Muscle weakness
Low blood pressure
Loss of appetite
Nausea

Cortisol blood test vs cortisol saliva test
GetTested’s Cortisol Test measures your cortisol levels from your saliva instead of blood, making it a lot more convenient to use. For example, people with naturally cold hands may experience difficulty acquiring blood from pricking their finger, which makes a blood test not ideal. It is also more comfortable for people who are sensitive to blood and needles. 
Testing cortisol throughout the day
GetTested’s Cortisol Test measures bioavailable and free cortisol at seven points throughout the day, combining the first morning values into one, resulting in five reported values. Cortisol is released in response to physical or mental stress, enhancing physical and mental performance under short-term stress. However, consistently high cortisol levels can be harmful, while low levels can also pose health problems. This test is especially useful for those experiencing chronic stress, fatigue, or other stress-related symptoms.
For a comprehensive hormonal assessment, DHEA—another hormone affected by stress—is often measured alongside cortisol. Our DHEA Saliva Test can provide additional insights into your stress response and hormonal balance.

About Cortisol 
Cortisol is a stress hormone that is released when we are exposed to physical or mental stress. Cortisol is produced in the adrenal glands and helps you perform better physically and mentally in situations that the body perceives as acute stress. In the distant past, stress was a natural reaction to protect yourself from predators, whereas nowadays stress takes on a different form. Today, our stress comes from a modern setting, such as stress about paying the bills. 
  
The stress we experience in today’s society often becomes chronic stress. This means high levels of cortisol building up in the body for long periods of time. This causes high levels of cortisol which is harmful and dangerous to the body, resulting in high blood pressure and high blood sugar which can cause type-2 diabetes. 
  
However, too little cortisol in the body is not good either. If the cortisol levels become really low, the urine excretes large amounts of sodium, which can lead to life-threatening conditions.  
  
It is important to know if you are at a risk of being affected by any of these conditions. By taking GetTested’s Cortisol test, you can get an indication of your cortisol levels and take adequate measures to prevent cortisol disorders.  
The function of cortisol 
Cortisol has a variety of functions in our body. In acute stressful situations, cortisol secretion helps you perform better, both physically and mentally. Cortisol is also important for the movement of the intestines and controls the body’s metabolism of sugar, fat and protein. With its anti-inflammatory effect, cortisol counteracts the function of the immune system, which makes us more susceptible to infections. 
Your cortisol level is not always related to your stress level. Physical activity also increases cortisol secretion in the body, but unlike secretion during stress, cortisol instead reduces inflammation that occurs on the muscles. In the medical world, cortisol is best known for its anti-inflammatory and muscle-degrading effects in the treatment of inflammatory diseases, and this is then treated by adding cortisone. 
Cortisol also controls the immune system and reduces swelling in allergies and insect bites. Therefore, Cortisol is a vital hormone for our wellbeing.  
Cortisol fluctuations  
Cortisol levels should be at the highest in the morning and lowest at night. The metabolism of fats, sugars and proteins is all controlled by the cortisol.  The amount of cortisol itself depends on what you do and how much stress you put on yourself. Stress can raise the cortisol levels, as well as being injured, having fever, infections or too high adrenaline.  
Symptoms of high cortisol: 
In the long run, however, high levels are harmful to the body and with high levels of cortisol for a long time, fat burning decreases and impairs insulin sensitivity, which in the long run can lead to obesity and type-2 diabetes.
Fatigue 
Low libido 
Weight gain 
Difficulty sleeping 
High blood pressure 
Poor immune function
Symptoms of low cortisol: 
Low levels of cortisol can lead to menstrual disorders, muscle weakness and general mental illness, as well as cortisol inhibiting inflammation. In more rare and extreme cases, low cortisol can lead to adrenal insufficiency (also known as Addison’s disease). In such cases, the body does not make enough hormones. Diseases like Addison’s disease can cause life-threatening conditions and usually need to be medicated, but “Milder” conditions still cause a huge stress on the body and mind and should not be left untreated. 
The most common symptoms of low cortisol are: 
Menstrual disorders 
Fatigue 
Muscle weakness 
Low blood pressure 
Loss of appetite 
Nausea
Cortisol blood test vs cortisol saliva test 
GetTested’s Cortisol Test measures your cortisol levels from your saliva instead of blood, making it a lot more convenient to use. For example, people with naturally cold hands may experience difficulty acquiring blood from pricking their finger, which makes a blood test not ideal. It is also more comfortable for people who are sensitive to blood and needles.  
Testing cortisol throughout the day 
GetTested’s Cortisol Test measures bioavailable and free cortisol at seven points throughout the day, combining the first morning values into one, resulting in five reported values. Cortisol is released in response to physical or mental stress, enhancing physical and mental performance under short-term stress. However, consistently high cortisol levels can be harmful, while low levels can also pose health problems. This test is especially useful for those experiencing chronic stress, fatigue, or other stress-related symptoms. 
For a comprehensive hormonal assessment, DHEA—another hormone affected by stress—is often measured alongside cortisol. Our DHEA Saliva Test can provide additional insights into your stress response and hormonal balance.

Cortisol Test

Men's Hormone Test: Hormones Analyzed
Testosterone, the primary male sex hormone, is essential for regulating libido, muscle mass, and bone density. Cortisol, known as the stress hormone, reflects the body's response to stress and impacts energy levels and immune function. DHEA, a precursor to sex hormones, plays a role in producing other essential hormones. Progesterone, although typically considered a female hormone, is important in men for balancing the effects of estrogen and maintaining a healthy prostate. Lastly, Estrogen (specifically estradiol, E2), often associated with female reproductive health, is also present in men and vital for healthy bones and emotional well-being.
DHEA's Role and Importance
Dehydroepiandrosterone (DHEA), or androstenolone, converts into both testosterone and estrogen. Recognized as a powerful hormone, it enhances performance in high doses. DHEA peaks in our 20s, then declines post-30. It influences mental and physical performance, energy, memory, muscle mass, skin health, body odor, bone mass, and puberty development, contributing to youthfulness and health.
Testosterone's Functions
Testosterone fuels energy, endurance, muscle strength, and libido. Low testosterone in men can lead to erectile dysfunction, reduced muscle strength, anxiety, depression, and low energy. Conversely, excessive testosterone might cause aggression.
Understanding Cortisol Levels
This test measures your morning cortisol levels. For a full-day cortisol profile, choose the Cortisol Test, taken 7 times a day. Cortisol, produced in the adrenal glands during stress, enhances physical and mental performance in acute stress situations. Both excessively high and low levels are problematic.
Progesterone in Men
Men require progesterone for testosterone production. Low progesterone can lead to symptoms like low libido, reduced sperm quality, depression, decreased muscle mass, fatigue, and poor memory, also negatively impacting sleep.
Measuring Estrogen in Men
Men have estrogen in smaller amounts. High estrogen levels can cause gynecomastia, erectile dysfunction, infertility, depression, and headaches/migraines.
How the Men's Hormone Test Works
You perform this saliva test at home twice in one day. Take the first sample immediately upon waking and the second 30 minutes later. Send the samples to our lab for analysis. You'll receive digital results.

Men's Hormone Test: Hormones Analyzed 
Testosterone, the primary male sex hormone, is essential for regulating libido, muscle mass, and bone density. Cortisol, known as the stress hormone, reflects the body's response to stress and impacts energy levels and immune function. DHEA, a precursor to sex hormones, plays a role in producing other essential hormones. Progesterone, although typically considered a female hormone, is important in men for balancing the effects of estrogen and maintaining a healthy prostate. Lastly, Estrogen (specifically estradiol, E2), often associated with female reproductive health, is also present in men and vital for healthy bones and emotional well-being. 
DHEA's Role and Importance 
Dehydroepiandrosterone (DHEA), or androstenolone, converts into both testosterone and estrogen. Recognized as a powerful hormone, it enhances performance in high doses. DHEA peaks in our 20s, then declines post-30. It influences mental and physical performance, energy, memory, muscle mass, skin health, body odor, bone mass, and puberty development, contributing to youthfulness and health. 
Testosterone's Functions 
Testosterone fuels energy, endurance, muscle strength, and libido. Low testosterone in men can lead to erectile dysfunction, reduced muscle strength, anxiety, depression, and low energy. Conversely, excessive testosterone might cause aggression. 
Understanding Cortisol Levels 
This test measures your morning cortisol levels. For a full-day cortisol profile, choose the Cortisol Test, taken 7 times a day. Cortisol, produced in the adrenal glands during stress, enhances physical and mental performance in acute stress situations. Both excessively high and low levels are problematic. 
Progesterone in Men 
Men require progesterone for testosterone production. Low progesterone can lead to symptoms like low libido, reduced sperm quality, depression, decreased muscle mass, fatigue, and poor memory, also negatively impacting sleep. 
Measuring Estrogen in Men 
Men have estrogen in smaller amounts. High estrogen levels can cause gynecomastia, erectile dysfunction, infertility, depression, and headaches/migraines. 
How the Men's Hormone Test Works 
You perform this saliva test at home twice in one day. Take the first sample immediately upon waking and the second 30 minutes later. Send the samples to our lab for analysis. You'll receive digital results.

Men’s Hormone Test

Urine Tests

The Organic Acids Profile Test (Large) from GetTested provides a broad assessment of 62 organic acids, offering a detailed overview of biochemical patterns in the body. Organic acids are natural byproducts of metabolism, and measuring them can give valuable insights into areas such as energy production, nutrient utilization, neurotransmitter metabolism, and detoxification pathways. This test is designed to support greater awareness of your body&rsquo;s metabolic balance and overall wellness.

Saliva Tests

GetTested&rsquo;s Cortisol Test measures bioavailable and free cortisol on seven occasions during a day. Cortisol is released in response to physical or mental stress. Produced by the adrenal glands, cortisol boosts physical and mental performance under short-term stress. However, consistently high cortisol levels can harm your health, while too low levels can also be problematic. This test is particularly useful for individuals experiencing chronic stress, fatigue, or other stress-related symptoms.
Additionally, DHEA, another hormone influenced by stress, is commonly measured alongside cortisol for a comprehensive hormonal assessment. Our&nbsp;<a href="/product/dhea-test/">DHEA Saliva Test</a>&nbsp;can further enhance understanding of your stress response and hormonal balance.

Men&rsquo;s Hormone Test by GetTested, is for those seeking an in-depth understanding of their hormonal health. This saliva-based test analyzes key hormones such as testosterone, DHEA, cortisol, estrogen, and progesterone. Tailored for men, it offers crucial insights into both sex hormones and adrenal hormones. Understanding these hormone levels can be vital in assessing various health aspects, including energy levels, mood stability, and overall physical well-being.
For those looking to further enhance their health screening, our PSA Test, available as both a&nbsp;<a href="/product/psa-test-rapid">rapid</a>&nbsp;and&nbsp;<a href="/product/parasite-test-rapid/">laboratory</a>&nbsp;test, is another popular option among men for monitoring prostate health.

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Cortisol & Stress Response | Key Functions | GetTested

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Cortisol, produced by adrenal glands, regulates metabolism, immune responses, and the body’s stress reactions. It's crucial for various physiological processes.

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Individuals experiencing chronic stress, fatigue, anxiety, or symptoms of adrenal dysfunction would benefit from cortisol testing. It&rsquo;s also helpful for those with irregular sleep patterns, weight changes, or ongoing health issues linked to stress.

Who would benefit from testing their cortisol levels?

High Cortisol:
<ul>
 <li>
 <ul>
 <li>Weight gain, especially in the abdomen</li>
 <li>Insomnia</li>
 <li>Anxiety and depression</li>
 <li>High blood pressure</li>
 <li>Muscle weakness</li>
 </ul>
 </li>
</ul>
Low Cortisol:
<ul>
 <li>
 <ul>
 <li>Chronic fatigue</li>
 <li>Muscle weakness</li>
 <li>Weight loss</li>
 <li>Low blood pressure</li>
 <li>Mood swings</li>
 </ul>
 </li>
</ul>

What are symptoms of low or high cortisol levels?

Saliva testing for cortisol measures free, active hormone levels, reflecting the body&rsquo;s immediate response to stress. Blood tests measure total cortisol, including both bound and unbound cortisol, giving a broader view of adrenal function over time.

 What is the difference between cortisol in saliva and blood?

Cortisol levels fluctuate throughout the day, peaking in the morning and gradually declining. Measuring several times captures this natural rhythm, providing a more accurate assessment of adrenal function and stress response.

Cortisol (free)

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