Hormones

Testosterone is a hormone that plays a crucial role in both male and female health. In men, it is primarily produced in the testes and is vital for the development of male reproductive tissues, promoting muscle growth, body hair, libido, and mood regulation. In women, testosterone is produced in smaller quantities by the ovaries and adrenal glands, where it supports bone strength, ovarian function, and sexual desire.

Testosterone (total)

Estrogen is a primary sex hormone found in both women and men, though at varying levels. In women, it supports the development and regulation of the reproductive system, menstrual cycle, and secondary sexual characteristics, while also affecting bone health, skin, and mood. In men, it contributes to libido, erectile function, and sperm production. It is primarily produced in the ovaries for women and the testes for men. Estrogen levels naturally fluctuate during puberty, menstrual cycles, pregnancy, and menopause. Imbalances can lead to menstrual irregularities, mood changes, and other health issues.

Estrogen (estradiol)

Free Thyroxine (fT4) is a vital thyroid hormone produced by the thyroid gland, playing a crucial role in metabolism, brain development, and bone health. It acts as the precursor to the active hormone fT3. Measuring fT4 offers insight into thyroid function by reflecting the hormone that is directly released and available for conversion.

Free T4 (fT4)

Free Triiodothyronine (fT3) is a measurement that indicates the level of the active thyroid hormone available in the body. As one of the two main thyroid hormones, fT3 plays a crucial role in regulating metabolism, heart rate, and body temperature. Unlike total T3, fT3 represents the unbound hormone that is immediately available for use, making it an important marker of thyroid function.

Free T3 (fT3)

DHEA-S (dehydroepiandrosterone sulfate) is the more stable and abundant form of DHEA found in the blood. Measuring DHEA-S levels provides a longer-term indicator of DHEA status, aiding in the assessment of hormonal balance, adrenal gland function, and age-related hormonal changes.

DHEA-S

Melatonin is a hormone mainly produced by the pineal gland in the brain. It plays a crucial role in regulating the body’s internal clock, including the sleep-wake cycle. Melatonin levels naturally increase in the evening, reach their peak overnight, and decrease by morning, helping signal the body to get ready for rest. It also supports other functions such as immune response and antioxidant protection.

Melatonin

Luteinizing Hormone (LH) is a hormone produced by the anterior pituitary gland that plays a key role in reproductive health for both men and women. In women, LH triggers ovulation and supports the menstrual cycle by stimulating progesterone production. In men, it promotes testosterone production in the testes. Maintaining balanced LH levels is important for proper hormonal regulation and reproductive function.

Luteinizing Hormone

DHEA (dehydroepiandrosterone) is a hormone produced by the adrenal glands and serves as a precursor to sex hormones. Measuring DHEA in saliva reflects the body’s active hormone levels, offering insight into immediate DHEA production. This measurement is helpful for evaluating adrenal health, aging, and the risk of chronic diseases.

DHEA

The estrogen/progesterone (E/P) ratio is a measurement that indicates the balance between two essential hormones in the body — estrogen (E) and progesterone (P). Both hormones are crucial for female reproductive health, including the regulation of menstrual cycles, fertility, mood, and overall wellness. Maintaining an optimal ratio promotes hormonal balance, while imbalances may lead to various health concerns. This ratio naturally varies throughout the menstrual cycle and can be affected by factors such as age, lifestyle, and medical conditions.

Estrogen/Progesterone ratio

Prolactin is a hormone produced by the pituitary gland primarily associated with lactation in women. It also plays key roles in fertility, immune system regulation, and sexual satisfaction. Elevated prolactin levels can cause symptoms such as irregular menstrual cycles or infertility, while low levels may affect milk production after childbirth.

Prolactin

Progesterone is a hormone that plays a crucial role in the female reproductive system, mainly produced in the ovaries after ovulation. It prepares the uterus for pregnancy and helps sustain it if conception occurs, while a decline in progesterone triggers menstruation. Beyond reproduction, progesterone affects sexual desire, mood, and bone health. During pregnancy, the placenta produces progesterone to support fetal development and prepare for breastfeeding. In men, progesterone is present at lower levels and contributes to sperm production.

Progesterone

The Testosterone/Cortisol ratio reflects the balance between anabolic (building) and catabolic (breaking down) processes in the body. Testosterone supports muscle growth, energy, and recovery, while cortisol is a stress hormone that can promote tissue breakdown when chronically elevated. A healthy ratio may indicate good resilience, recovery capacity, and hormonal balance, whereas a low ratio may suggest high stress load, overtraining, or hormonal imbalance. This marker is particularly relevant for athletes, individuals under chronic stress, or those with fatigue-related symptoms. It helps provide context to both testosterone and cortisol values when interpreted together.

Testosterone/Cortisol ratio

Thyroid Stimulating Hormone (TSH) is a hormone produced by the pituitary gland that regulates thyroid function. It signals the thyroid gland to produce thyroid hormones, which are essential for metabolism, energy regulation, and growth. TSH levels provide important information for diagnosing thyroid disorders and assessing whether the thyroid is functioning properly.

Free testosterone refers to the fraction of testosterone in the bloodstream that is not bound to proteins like sex hormone-binding globulin (SHBG) or albumin. Unlike bound testosterone, free testosterone is biologically active and readily available to interact with cells, making it a key indicator of hormonal activity in the body. It plays a vital role in regulating libido, energy levels, muscle strength, and mood in both men and women.

Testosterone (free)

Sex hormone-binding globulin (SHBG) is a glycoprotein primarily produced by the liver that binds and transports sex steroids—mainly testosterone and estradiol—in the bloodstream. By binding these hormones, SHBG controls how much is biologically available to tissues. Higher SHBG levels typically decrease the free (unbound) fraction of testosterone and estradiol, while lower SHBG levels increase it. Due to this regulatory function, SHBG plays a key role in hormonal balance for both men and women and is often measured alongside total testosterone and estradiol to help with interpretation.

Sex hormone-binding globulin (SHBG)

Cortisol is the primary glucocorticoid hormone produced by the adrenal glands and is commonly known as the “stress hormone.” It plays a vital role in regulating metabolism, immune response, blood pressure, and the body’s ability to handle physical and emotional stress. Cortisol follows a daily cycle, peaking early in the morning to help promote alertness and gradually decreasing throughout the day. Measuring total cortisol in the blood reflects the combined levels of free and protein-bound cortisol, offering insight into adrenal function and overall hormone balance. Abnormal cortisol levels may be linked to conditions such as adrenal insufficiency, Cushing’s syndrome, chronic stress, or metabolic disorders.

Cortisol (total)

Follicle-Stimulating Hormone (FSH) is a hormone produced by the pituitary gland that plays a crucial role in reproduction for both men and women. In women, FSH prompts the ovaries to mature egg follicles, while in men, it supports sperm production. FSH levels fluctuate throughout a woman’s menstrual cycle and are vital for fertility and overall reproductive health.

Free Cortisol refers to the unbound, biologically active form of cortisol circulating in the body. Unlike total cortisol, which includes both bound and unbound hormone, free cortisol represents the fraction that is available to tissues and can directly influence stress response, metabolism, immune function, and energy levels. Measuring free cortisol provides a more accurate reflection of how the body is responding to physical and emotional stress. It is commonly assessed in urine or saliva samples and is particularly relevant in evaluating adrenal function and circadian rhythm imbalances.

Cortisol (free)

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Men's Hormone Test: Hormones Analyzed
Testosterone, the primary male sex hormone, is essential for regulating libido, muscle mass, and bone density. Cortisol, known as the stress hormone, reflects the body's response to stress and impacts energy levels and immune function. DHEA, a precursor to sex hormones, plays a role in producing other essential hormones. Progesterone, although typically considered a female hormone, is important in men for balancing the effects of estrogen and maintaining a healthy prostate. Lastly, Estrogen (specifically estradiol, E2), often associated with female reproductive health, is also present in men and vital for healthy bones and emotional well-being.
DHEA's Role and Importance
Dehydroepiandrosterone (DHEA), or androstenolone, converts into both testosterone and estrogen. Recognized as a powerful hormone, it enhances performance in high doses. DHEA peaks in our 20s, then declines post-30. It influences mental and physical performance, energy, memory, muscle mass, skin health, body odor, bone mass, and puberty development, contributing to youthfulness and health.
Testosterone's Functions
Testosterone fuels energy, endurance, muscle strength, and libido. Low testosterone in men can lead to erectile dysfunction, reduced muscle strength, anxiety, depression, and low energy. Conversely, excessive testosterone might cause aggression.
Understanding Cortisol Levels
This test measures your morning cortisol levels. For a full-day cortisol profile, choose the Cortisol Test, taken 7 times a day. Cortisol, produced in the adrenal glands during stress, enhances physical and mental performance in acute stress situations. Both excessively high and low levels are problematic.
Progesterone in Men
Men require progesterone for testosterone production. Low progesterone can lead to symptoms like low libido, reduced sperm quality, depression, decreased muscle mass, fatigue, and poor memory, also negatively impacting sleep.
Measuring Estrogen in Men
Men have estrogen in smaller amounts. High estrogen levels can cause gynecomastia, erectile dysfunction, infertility, depression, and headaches/migraines.
How the Men's Hormone Test Works
You perform this saliva test at home twice in one day. Take the first sample immediately upon waking and the second 30 minutes later. Send the samples to our lab for analysis. You'll receive digital results.

Men's Hormone Test: Hormones Analyzed 
Testosterone, the primary male sex hormone, is essential for regulating libido, muscle mass, and bone density. Cortisol, known as the stress hormone, reflects the body's response to stress and impacts energy levels and immune function. DHEA, a precursor to sex hormones, plays a role in producing other essential hormones. Progesterone, although typically considered a female hormone, is important in men for balancing the effects of estrogen and maintaining a healthy prostate. Lastly, Estrogen (specifically estradiol, E2), often associated with female reproductive health, is also present in men and vital for healthy bones and emotional well-being. 
DHEA's Role and Importance 
Dehydroepiandrosterone (DHEA), or androstenolone, converts into both testosterone and estrogen. Recognized as a powerful hormone, it enhances performance in high doses. DHEA peaks in our 20s, then declines post-30. It influences mental and physical performance, energy, memory, muscle mass, skin health, body odor, bone mass, and puberty development, contributing to youthfulness and health. 
Testosterone's Functions 
Testosterone fuels energy, endurance, muscle strength, and libido. Low testosterone in men can lead to erectile dysfunction, reduced muscle strength, anxiety, depression, and low energy. Conversely, excessive testosterone might cause aggression. 
Understanding Cortisol Levels 
This test measures your morning cortisol levels. For a full-day cortisol profile, choose the Cortisol Test, taken 7 times a day. Cortisol, produced in the adrenal glands during stress, enhances physical and mental performance in acute stress situations. Both excessively high and low levels are problematic. 
Progesterone in Men 
Men require progesterone for testosterone production. Low progesterone can lead to symptoms like low libido, reduced sperm quality, depression, decreased muscle mass, fatigue, and poor memory, also negatively impacting sleep. 
Measuring Estrogen in Men 
Men have estrogen in smaller amounts. High estrogen levels can cause gynecomastia, erectile dysfunction, infertility, depression, and headaches/migraines. 
How the Men's Hormone Test Works 
You perform this saliva test at home twice in one day. Take the first sample immediately upon waking and the second 30 minutes later. Send the samples to our lab for analysis. You'll receive digital results.

Men’s Hormone Test

The Role of Hormones
Progesterone, estrogen, testosterone, DHEA, and cortisol each play unique roles in the body, affecting everything from brain function to stress response.
Who Should Take This Test?
This test is ideal if you want a comprehensive view of your hormones, including cortisol. Men should opt for the Men's Hormone Test.
Common Symptoms of Hormonal Imbalances
You might experience mood swings, depression, PMS, irregular periods, PCOS, infertility, menopausal symptoms, endometriosis, low libido, tender breasts, heavy periods, brain fog, impaired memory, weight gain, osteoporosis, vaginal dryness, headaches, and loose stools before and during menstruation, among other symptoms.
DHEA: A Versatile HormoneDehydroepiandrosterone (DHEA), or androstenolone, is a hormone that converts into both testosterone and estrogen. It's so potent that excessive doses classify as performance-enhancing drugs. DHEA peaks in our 20s and then gradually declines post-30. It boosts mental and physical performance, energy, memory, muscle mass, skin quality, body odor, bone mass, and puberty development, while keeping us young and healthy.
Cortisol: The Stress HormoneOur test measures your morning cortisol levels. For diurnal cortisol measurement, we recommend the Cortisol Test, taken 7 times throughout the day. Cortisol, a stress hormone from the adrenal glands, boosts physical and mental performance in stressful situations. Both high and low levels can be problematic.
Estrogen: Key to FertilityThe Women's Hormone Test measures estradiol (E2) and its ratio to progesterone. Estradiol, the most crucial estrogen, is known as the fertility hormone in women.
Progesterone: More Than a Sex HormoneProgesterone, primarily a female sex hormone, influences fertility and is a precursor to sex hormones testosterone, estrogen, and cortisol. It's vital for the central nervous system. Its levels fluctuate during the menstrual cycle and increase post-ovulation.
Testosterone in WomenThough often seen as a male hormone, women need testosterone for energy, libido, and muscle building. Women with PCOS often have higher testosterone levels.
How to Take the Women's Hormone Test
Perform this saliva test at home by taking two samples: one immediately after waking up and another 30 minutes later. If menstruating, collect samples a week post-ovulation, around day 21. In or post-menopause, timing is more flexible. After collection, send the samples to our lab. You will receive your results online.

The Role of Hormones 
Progesterone, estrogen, testosterone, DHEA, and cortisol each play unique roles in the body, affecting everything from brain function to stress response. 
Who Should Take This Test? 
This test is ideal if you want a comprehensive view of your hormones, including cortisol. Men should opt for the Men's Hormone Test. 
Common Symptoms of Hormonal Imbalances 
You might experience mood swings, depression, PMS, irregular periods, PCOS, infertility, menopausal symptoms, endometriosis, low libido, tender breasts, heavy periods, brain fog, impaired memory, weight gain, osteoporosis, vaginal dryness, headaches, and loose stools before and during menstruation, among other symptoms. 
DHEA: A Versatile HormoneDehydroepiandrosterone (DHEA), or androstenolone, is a hormone that converts into both testosterone and estrogen. It's so potent that excessive doses classify as performance-enhancing drugs. DHEA peaks in our 20s and then gradually declines post-30. It boosts mental and physical performance, energy, memory, muscle mass, skin quality, body odor, bone mass, and puberty development, while keeping us young and healthy. 
Cortisol: The Stress HormoneOur test measures your morning cortisol levels. For diurnal cortisol measurement, we recommend the Cortisol Test, taken 7 times throughout the day. Cortisol, a stress hormone from the adrenal glands, boosts physical and mental performance in stressful situations. Both high and low levels can be problematic. 
Estrogen: Key to FertilityThe Women's Hormone Test measures estradiol (E2) and its ratio to progesterone. Estradiol, the most crucial estrogen, is known as the fertility hormone in women. 
Progesterone: More Than a Sex HormoneProgesterone, primarily a female sex hormone, influences fertility and is a precursor to sex hormones testosterone, estrogen, and cortisol. It's vital for the central nervous system. Its levels fluctuate during the menstrual cycle and increase post-ovulation. 
Testosterone in WomenThough often seen as a male hormone, women need testosterone for energy, libido, and muscle building. Women with PCOS often have higher testosterone levels. 
How to Take the Women's Hormone Test 
Perform this saliva test at home by taking two samples: one immediately after waking up and another 30 minutes later. If menstruating, collect samples a week post-ovulation, around day 21. In or post-menopause, timing is more flexible. After collection, send the samples to our lab. You will receive your results online.

Women’s Hormone Test

Saliva Tests

Men&rsquo;s Hormone Test by GetTested, is for those seeking an in-depth understanding of their hormonal health. This saliva-based test analyzes key hormones such as testosterone, DHEA, cortisol, estrogen, and progesterone. Tailored for men, it offers crucial insights into both sex hormones and adrenal hormones. Understanding these hormone levels can be vital in assessing various health aspects, including energy levels, mood stability, and overall physical well-being.
For those looking to further enhance their health screening, our PSA Test, available as both a&nbsp;<a href="/product/psa-test-rapid">rapid</a>&nbsp;and&nbsp;<a href="/product/parasite-test-rapid/">laboratory</a>&nbsp;test, is another popular option among men for monitoring prostate health.

Women&rsquo;s Hormone Test analyses cortisol, estrogen, progesterone, testosterone, DHEA, and the ratio between estrogen and progesterone. Many women today face challenges with PMS, infertility, fatigue, irregular periods, PCOS, menopausal symptoms, and more. This test offers a detailed understanding of both your sex hormones and adrenal hormones, providing insights into various health issues.
Additionally, if you are experiencing mood issues, we recommend our&nbsp;<a href="/product/neurotransmitters-basic">Neurotransmitters Basic</a>&nbsp;or&nbsp;<a href="/product/neurotransmitters-plus">Plus</a>&nbsp;tests, which measure levels of key neurotransmitters such as serotonin and dopamine, to further explore potential imbalances affecting your well-being.

Urine Tests

The Organic Acids Profile Test (Large) from GetTested provides a broad assessment of 62 organic acids, offering a detailed overview of biochemical patterns in the body. Organic acids are natural byproducts of metabolism, and measuring them can give valuable insights into areas such as energy production, nutrient utilization, neurotransmitter metabolism, and detoxification pathways. This test is designed to support greater awareness of your body&rsquo;s metabolic balance and overall wellness.

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Cortisol & Stress Response | Key Functions | GetTested

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Cortisol, produced by adrenal glands, regulates metabolism, immune responses, and the body’s stress reactions. It's crucial for various physiological processes.

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Individuals experiencing chronic stress, fatigue, anxiety, or symptoms of adrenal dysfunction would benefit from cortisol testing. It&rsquo;s also helpful for those with irregular sleep patterns, weight changes, or ongoing health issues linked to stress.

Who would benefit from testing their cortisol levels?

High Cortisol:
<ul>
 <li>
 <ul>
 <li>Weight gain, especially in the abdomen</li>
 <li>Insomnia</li>
 <li>Anxiety and depression</li>
 <li>High blood pressure</li>
 <li>Muscle weakness</li>
 </ul>
 </li>
</ul>
Low Cortisol:
<ul>
 <li>
 <ul>
 <li>Chronic fatigue</li>
 <li>Muscle weakness</li>
 <li>Weight loss</li>
 <li>Low blood pressure</li>
 <li>Mood swings</li>
 </ul>
 </li>
</ul>

What are symptoms of low or high cortisol levels?

Saliva testing for cortisol measures free, active hormone levels, reflecting the body&rsquo;s immediate response to stress. Blood tests measure total cortisol, including both bound and unbound cortisol, giving a broader view of adrenal function over time.

 What is the difference between cortisol in saliva and blood?

Cortisol levels fluctuate throughout the day, peaking in the morning and gradually declining. Measuring several times captures this natural rhythm, providing a more accurate assessment of adrenal function and stress response.

Cortisol (free)

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